two-way analysis of variance (anova) followed by tukeys multiple comparisons test Search Results


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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by <t>a</t> <t>two-way</t> <t>ANOVA</t> followed by Tukey’s multiple comparison test.
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Image Search Results


Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by a two-way ANOVA followed by Tukey’s multiple comparison test.

Journal: Plant Signaling & Behavior

Article Title: MicroRNA858a antagonistically regulates plant response to concurrent biotic and abiotic stresses

doi: 10.1080/15592324.2026.2670872

Figure Lengend Snippet: Relative expression analysis of transcription factors regulating miR858a in response to flg22, UV-B, and co-treatment F/U. (A) TF candidates upregulated by flg22 but downregulated by UV-B. (B) TF candidates downregulated by flg22 but upregulated by UV-B. (C) TF candidates upregulated by both flg22 and UV-B. (D) TF candidates were downregulated by both flg22 and UV-B. (E) TF candidates specifically respond to flg22. The relative expression level is based on the comparative Ct method. Data were obtained from three independent biological samples and represent the mean ± SE. Normalization of the expression levels was carried out using Actin2 as an internal reference gene. Statistically significant differences between the mock control levels (CK) and treatment transcripts were determined by a two-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Multiple comparisons of statistical significance were carried out using a two-way ANOVA followed by Tukey's multiple comparison test according to Minitab software.

Techniques: Expressing, Control, Comparison